Log in Subscribe

PH-Sensitive Chitosan Nanoparticles Debased With Dolutegravir As Milk And Food Admixture For Paediatric Anti-HIV Therapy

Kragelund Thuesen
· 3 min read
PH-Sensitive Chitosan Nanoparticles Debased With Dolutegravir As Milk And Food Admixture For Paediatric Anti-HIV Therapy

The present study aims to develop Chitosan-free-based polymeric nanoparticles of anti-HIV drug Dolutegravir, to aid appropriate dose adjustment and ease of oral administration as milk and food admixture for babys. The isolated Chitosan from the crab shell species Portunus Sanguinolentus has been qualifyed for their physicochemical holdings. Nanoparticles were developed with changing ratio of drug: Chitosan and assessed for particle size (140-548 nm), zeta potential (+26 mV) with a maximum of 75 % drug content. Nanoparticles paraded improved stability and drug release in the 0 N HCl medium compared to pure drug. The MTT assay and the Syncytia inhibition assay in C8166 (T-lymphatic cell line) infected with HIV(IIIB) viral strain, which expressed better therapeutic efficiency and lesser cytotoxicity equated to the pure drug. In consonance with the data obtained, the use of chitosan from a novel source for drug delivery carrier has unfolded exceptional panoramas for having drugs efficiently to paediatrics.

supervising freshness of chicken breast by expending natural halochromic curcumin laded chitosan/PEO nanofibers as an intelligent package.Intelligent packaging is important to get information about real time quality of foods. The objective of this study was to develop an electrospun nanofiber halochromic pH sensor film employing curcumin, chitosan (CS) and polyethylene oxide (PEO) to monitor chicken freshness. Conductivity and rheological behavior of CS/PEO/curcumin resolutions were measured to understand the effect of solution dimensions on the morphology of the fibers. The morphological characteristics of nanofiber films were inquired by Field Emission Scanning Electron Microscopy (FESEM). Average diameter of the fibres was found to be between 283 ± 27 nm and 338 ± 35 nm. It was reasoned that increasing CS amount in nanofibers diminished the diameter of the fibres.

Thermal analysis and water vapor permeability features of the pH sensor were also tested. Color alterations of curcumin laded CS/PEO nanofiber film was appraised on chicken breast package at 4 °C. The color of nanofiber film shifted from bright yellow to reddish color which leaved an opportunity to detect color varietys by even the naked eyes of the untrained consumer. As a quality indicator, surface pH varietys of the chicken breast and TVB-N (total volatile basic nitrogen) were appraised. At the end of the day 5, pH value of 6 ±0 and TVB-N concentration of 23 ±3 mg/100 g suggested that food was at the edge of the acceptance level. As a result, curcumin charged nanofiber fulfilled the expectation and gave an opportunity to visualize real time monitoring of chicken spoilage.Shelf-life extension of pomegranate arils employing chitosan nanoparticles debased with Satureja hortensis essential oil.

BACKGROUND: This study was imparted in two partings to improve the antimicrobial activity and stability of Satureja hortensis essential oil (SEO) and its wallops on the quality of pomegranate arils. In the first part, SEO was capsulised by an ionic gelation technique into 142-267 nm chitosan nanoparticles (CSNPs). In  fucose structure  of the experiment, the CSNPs and CSNPs-SEO were practiced to improve storability of pomegranate arils. Arils were dimed in water (control), CSNPs and CSNPs-SEO for 5 min. After superficial water removal, arils were jamed into polystyrene boxwoods and stored at 5 °C for 18 days Based on spectrophotometry analysis, the encapsulation efficiency (EE) of SEO-charged CSNPs (CSNPs-SEO) decreased from 26% to 7% and their loading capacity (LC) increased from 4% to 6%, respectively, upon increasing the initial SEO content from 0 to 0 g g(-1) of chitosan. Phytochemicals and water content were wielded, and microbial enumerations were contracted in the coated arils during storage. Total phenol and antioxidant activity decreased during storage.

At the end of storage, the highest total phenol content (2980 mg gallic acid eqs L(-1) ) was noticed in arils processed with CSNPs-SEO, whereas ascorbic acid content was maximal (6 mg L(-1) ) in arils addressed with CSNPs.