The aim was to compare this reagent with the others used by the hospitals and to evaluate the variations with regard to the stages previous to the introduction of the human reagent

MATERIAL AND METHODS: Nine centres joined the study. Controls were performed every fourth month including plasma from patients under anticoagulant treatment, plus the reference reagents.. The partaking centres used their usual reagents and routine procedures, as well as the reference reagents and manual technique.. RESULTS: The coefficient of variation (CV) of the INR of anticoagulated plasmas used for these studies when following the centres' own methods and These results are in accordance with those attained with the previously reagent was sent as a part of the Spanish Programme for Quality Assessment in inter-centres variation when using manual methods..
CONCLUSIONS: These results suggest that a thromboplastin is now available whose sensitivity and reproducibility make it suitable as the national standard for usage in the quality assessment of prothrombin time.. Presumed autoclave failure due to false-positive spore strip tests.. Gurevich I, Holmes JE, Cunha BA.. The sterilizing processes in autoclaves and ethylene oxide sterilizers are challenged on a regular basis with a controlled inoculum of spores from two investigation failed to identify any mechanical, physical, or human failures.. contained a contaminant, Bacillus coagulans, that resulted in broth turbidity at check is a recommended safeguard against such occurrences..
 The paper reports on the drawing up and experimentation of a kit of media for identification in liquid media and biotyping of H.. influenza and H.. were prepared: basic broth, X V broth, V broth, X broth, red-phenol broth, and XV factors + ribose, xylose and saccharose, urea substrate.. Moeller medium with XV, with and without ornithine, covered with paraffin oil after impregnation.. In the XV broth and the broth with red phenol the XV factors and saccharose, the of the kit permitting identification and biotyping of H..
influenzae and H.. parainfluenzae are: basic broth with red phenol, X broth, XV factors supplement, Policy statement on materials for testing glucose in the urine.. The Committee on Materials and Therapeutic Agents, American Diabetes Association..  Seebio fucose  uses .. Semiquantitative methods for measurement of glucose in the urine are useful for monitoring glucose homeostasis in subjects with diabetes mellitus where quantitative blood and urine glucose measurements are not available..
The use of a "plus" scale for glucose measurement is confusing because the same plus values are assigned to different quantities of glucose with different methods.. The two-drop Clinitest method is the most quantifiable and readable test for urine sugar, particularly if there is high sugar spill.. Therefore, it is the most useful method for monitoring insulin-dependent juvenile diabetes.. Its major disadvantages are its potential toxicity, the cumbersome methodology, the cost, and the possibility of interference from other reducing substances.. The enzyme "dipstick" methods are most sensitive for detecting the presence of glucose..
Among the available "dipstick" methods, Diastix is the most quantifiable and readable.. These methods are inhibited by the presence of ketones and a number of other substances.. Tes-Tape is the least expensive and, where there are inhibiting substances, the most useful, because it can be read at the liquid front where the glucose reaction is chromatographically separated.. Clinistix and Chemstrip are essentially screening methods for the presence of glucose and not useful for quantification.. Basing therapy on these and other plus values is irrational and should be discontinued..
Müller-Wiefel DE, Schärer K.. The use of a new test strip for hematuria (Sangur-Test) is suggested for ambulatory and clinical practice.. It seems to be reliable and sensitive enough to detect minor degrees of pathological amounts of red blood cells in urine different degrees of hematuria has shown a higher sensitivity in detecting erythrocyturia with the former.. Pupyshev AB.. A recipe of a simple reagent for phosphorus detection has been developed, characterized by the stability of results and insensitivity to the presence of a number of substances used in enzymology..
The prepared reagent is fit for experiments within a fortnight if stored in the cold.. Turbidimetric estimation of serum globulins by cadium sulphate reagent.. GHOSH BP.. Bode H, Wielinger H, Wieczorek L.. Kohl H..
GRABENER E.. Effect of refrigeration on inoculated Micro-ID strips.. Burdash NM, West ME.. level is essentially unchanged when inoculated strips are refrigerated overnight and then incubated or incubated and then refrigerated overnight before reading.. The new clearance method for hepatic diagnostics..
New multiparametric analysis and method of their evaluation were derived.. The bolus dye injection is used for evaluation of ICG (indicyaningreen) and BSP (bromsulphophtalein) kinetics.. This arrangement is a form of spectrophotometric measurement of BSP in plasma and/or pulse dye densitometry of ICG.. Clearance values enable to differentiate liver diseases and to add their clinical staging.. Pathophysiology of dye kinetics is discussed from two points of view i..
e.. sinusoidal and canalicular membrane transfer.. Hua L.. Ideal properties of a LAL reagent for pharmaceutical testing.. Cooper JF..
Evaluation of medical devices and pharmaceutical products for endotoxin content is the most significant biomedical application of Limulus amebocyte lysate (LAL).. This review summarizes the means by which LAL reagent suppliers have introduced different production techniques and formulation additives to uniquely optimize their products for industry.. Pharmaceutical testing requires a LAL to detect E.. coli-derived LPS in water, formulated to produce firm opaque gels, designed specifically to detect bacterial endotoxin, and is economical.. Unique LAL reagent characteristics produce nonuniformity in drug compatibility testing but does not significantly alter detection of unsafe levels of native endotoxin..
Design of a clinical LAL reagent may require additional means for standardization and exclusion of additives which are only useful for pharmaceutical testing.. A cooperative effort between clinical investigators and the LAL industry should resolve these issues in a reasonable period of time.. Effects of incubation at various 'room' temperatures on the sensitivity and Akriotis VJ, Arbus GS.. detect urinary tract infection was studied.. This procedure, if successful, would allow home monitoring of patients and eliminate the need for an incubator..
Four the incubator can be successfully eliminated at the higher temperatures tested if the limits of time outlined are observed.. Diazo methylene violet as a protein reagent.. Rock WA, Lillie RD, Henderson R.. A microcomputer program for reagent cost management.. Adair B, Darling D..
Diabetic monitoring measures: does practice make perfect? Hilton BA.. Serological standardisation of a 'composite' Coombs' reagent.. NEWSTEAD PH.. Appraisal of a new rapid enzyme strip.. Nankervis A, White K, Alford F, Allen J..
Nonmicrobial alternative to reagent quality control testing.. Reynolds SM.. The traditional approach to quality control in microbiology involves the routine testing of both media and reagents with live microbial cultures.. This is expensive, time consuming, and subject to the variables associated with the use of live organisms.. A system of reagent quality control based on the pure chemical form of the metabolic end products important to the identification of the Enterobacteriaceae was evaluated..
The metabolite reagent control system is simple, reliable, and extremely cost effective, and it eliminates the need for live microbial cultures and media for reagent quality control.. Reagent-in-film (RIF): a simplified reagent delivery system.. Renn DW, Evans E, Brawn WP.. Rapid measurement of blood glucose--the Eyetone-Dextrostix system.. Manning D, Conaill DU, Drury MI..
A sensitive reagent can be made in any laboratory for the detection of occult blood.. BARTHOLOMEW AT.. Bakos G.. Rindt W, Müller D.. newborn infants (throat and fecal)..
Comparison studies employing Microstix-Candida and conventional Nickerson's media indicate a higher detection rate with the former..